A brief guide to pipette types Labmate Online

2021-11-25 07:54:25 By : Ms. Rita Xu

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This guide aims to explain the various uses of the most common types of pipettes in laboratories today. In a laboratory environment, pipettes are used to quickly and accurately transfer fluid from one container to another.

Although there are many different types of pipettes available, it is important to remember that some pipettes provide higher accuracy than others. Volumetric pipettes are still the most accurate in the world. The article Good Pipetting Techniques-Simple Ways to Minimize Errors discusses in more detail methods to obtain consistent pipetting results. 

Generally, those who study chemical properties and analyze reactions use volumetric pipettes. They can be found in most schools, universities and professional laboratories. They are known for their accuracy and can measure up to four significant digits. They are available in a variety of sizes, allowing researchers to measure the volume of concentrated stock solutions.

Graduated pipettes are not as accurate as volumetric pipettes. Moiré graduated pipettes, sometimes called "discharge pipettes", are marked with a zero at the beginning of their tapered end, while serological graduated pipettes, also called "blow-out pipettes", do not display Zero mark.

Vacuum assisted pipettes can be graduated or volumetric. Graduated vacuum-assisted pipettes use multiple scale marks, while volumetric vacuum-assisted pipettes measure a single volume, so only one scale mark is displayed. The vacuum-assisted pipette is made of polystyrene, glass or borosilicate. They require a suction device, but do not contain a piston.

Micropipettes enable scientists and technicians to obtain very accurate measurement results. Micropipettes should be calibrated regularly-at least every 3-6 months.

Pasteur pipettes are made of glass. The bulb-shaped top of the Pasteur pipette resembles a typical liquid dropper. Pasteur pipettes are considered quite inaccurate today. They are neither calibrated nor scaled, and are more commonly used in biology-not chemistry-laboratories as a way to transfer aqueous solutions from one container to another. Pasteur pipettes are named after the French doctor Louis Pasteur and are usually discarded after use.

To learn more about methods for measuring the liquid delivery performance of pipettes, please read this article: Traceable methods for verifying the performance of 96-well/384-well pipettes. Alternatively, you can view the list of pipette suppliers in the Labmate online supplier guide. 

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